PhD position - On-Chip moniToring of Organoids using an oPtoflUidiC systEm

OPPORTUNITY DETAILS

Total reward

0 $

State University

CEA Tech

Area

Western Europe

Host Country

France

Deadline

31 Oct 2021

Study level

Doctoral Degree

Opportunity type

PhD

Specialities

Engineering , Physics

Opportunity funding

Not funding

Eligible Countries

This opportunity is destined for all countries

Eligible Region

All Regions

SL-DRT-21-0534

RESEARCH FIELD

Health and environment technologies, medical devices

ABSTRACT

The thesis is positioned in the domain of organoids, which enable fundamental tissue mechanisms to be studied in-vitro. The project aims at developing a new microfluidic system with integrated simple, robust and compact optical reading, and allowing for in-situ monitoring of the secretome evolution during the cell culture. The chosen model system is the culture of islets of Langerhans responsible for the endocrine function of the pancreas and playing an important role in diabetes. To reach our objective, we propose to combine two approaches within a microfluidic chip: a chip with photonic-crystal sensors that are compatible with lens-less optical reading (LED + CMOS), and a microfluidics technology integrating the pneumatic actuation of an elastic membrane. The main challenges of the project are related to the sensors sensitivity, the optical chip integration in the microfluidic system and the sampling of aliquots without perturbation of the organoid. The thesis will be in collaboration between two complementary laboratory, CEA-LETI (microfluidics, technology), and INL (photonic biosensors).